NGS-QC Generator

The NGS-QC Generator is an essential software tool developed to address a crucial issue often underestimated in systems biology-based approaches. The combination of massive parallel sequencing with a variety of modern DNA/RNA enrichment technologies has provided means for interrogating functional protein-genome interactions (ChIP-seq), genome-wide transcriptional activity (RNA-seq; GRO-seq), chromatin accessibility (DNase-seq, FAIRE-seq, MNase-seq), and more recently, the three-dimensional organization of chromatin (Hi-C, ChIA-PET). However, the conclusions drawn from multidimensional analyses of NGS-derived datasets critically depend on the quality of the compared datasets. Any errors or inconsistencies during the sequencing can affect the accuracy of the results and render them useless.

The NGS-QC Generator software tool has been developed to overcome this problem. It is a quality control system that helps infer quality descriptors for any ChIP-sequencing and related datasets. The tool has been specifically designed to assess the quality of ChIP-seq datasets, which are widely used to investigate protein-DNA interactions, and RNA-seq datasets, which are used to study gene expression. By using the NGS-QC Generator, researchers can ensure that their datasets meet the required quality standards and can be used for further analysis.

The software tool provides detailed reports on the quality of the datasets. It generates quality descriptors that can be used to compare datasets and identify any potential errors or inconsistencies. The reports generated by the NGS-QC Generator are easy to interpret, and researchers can quickly identify any problem areas in their datasets.

One of the most significant advantages of the NGS-QC Generator is that it allows researchers to explore a vast database of QC indicators for over 21,000 publicly available datasets.